PLGA Microsphere-Mediated Growth Hormone Release Hormone Expression Induces Intergenerational Growth |
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| Authors: | Xiao-Hui Ren Hu-Ying Luo Hong-Yi Li Song-Cai Liu Ming-Jun Zhang |
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| Institution: | 1. College of Ocean, Heibei Agricultural University , Qinhuangdao, China;2. College of Animal Science and Veterinary Medicine, Jilin University , Changchun, China;3. College of Animal Science and Veterinary Medicine, Jilin University , Changchun, China;4. College of Animal Science, South China Agricultural University , Wushan, China;5. College of Ocean, Heibei Agricultural University , Qinhuangdao, China |
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| Abstract: | To improve animal growth, growth hormone-releasing hormone (GHRH) expression vectors that maintain constant GHRH expression can be directly injected into muscles. To deliver the GHRH expression vectors, biodegradable microspheres have been used as a sustained release system. Although administering GHRH through microspheres is a common practice, the intergenerational effects of this delivery system are unknown. To investigate the intergenerational effects of polylactic-co-glycolic acid (PLGA) encapsulated plasmid-mediated GHRH supplements, pCMV-Rep-GHRH microspheres were injected into pregnant mice. Growth and expression of GHRH were measured in the offspring. RT-PCR and immunohistochemistry reveal GHRH expression 3–21 days post-injection. The proportion of GH-positive cells in the GHRH treated offspring was 48.2% higher than in the control group (P < 0.01). The GHRH treated offspring were 6.15% (P < 0.05) larger than the control offspring. At day 49 post-injection, IGF-I serum levels were significantly higher in the treatment group than in the control group. This study confirms that intramuscular expression of GHRH mediated by PLGA microspheres significantly enhances intergenerational growth. |
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| Keywords: | Growth hormone Growth hormone-releasing hormone Intergenerational effects PLGA microspheres |
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