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Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles |
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| Authors: | Yasuhiro Morita Ni Wayan Kurniani Karja Lanh Thi Kim Do Fuminori Tanihara Masayasu Taniguchi |
| Institution: | 1. Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan;2. Division of Reproduction, Department of Clinic, Reproduction, and Pathology, Bogor Agriculture University, Indonesia |
| Abstract: | Fragmin/protamine microparticles (F/P MPs) approximately 0.5-1 µM in diameter were prepared by the simple mixing of fragmin with protamine. This study investigated the effects of F/P MP-containing collagen gels as a hormone carrier on the formation of antral follicle-like structures and on the development of growing bovine oocytes. The supplementation of F/P MPs in collagen gels contributed to the beneficial effects of follicle stimulating hormone (FSH) on the formation and size of antral follicle-like structures. The F/P MPs may serve as potential hormone carriers for the growth of cultured bovine oocytes from early antral follicles. |
| Keywords: | Follicular formation FSH In vitro culture Nanoparticles Oocyte |