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Association and in Silico Assignment of Sequences from Turkey BACs
Authors:Kent M. Reed  Gretchen M. Faile  Stacy B. Kreuth  Laura M. Sullivan  Lee D. Chaves
Affiliation:1. Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine , University of Minnesota , St. Paul, Minnesota, USA reedx054@umn.edu;3. Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine , University of Minnesota , St. Paul, Minnesota, USA;4. Department of Comparative Biosciences, School of Veterinary Medicine , University of Wisconsin-Madison , Madison, Wisconsin, USA
Abstract:Bacterial artificial chromosomes (BACs) provide an important resource in genetic mapping. An initial set of BACs corresponding to microsatellite markers in the turkey (Meleagris gallopavo) was isolated from the CHORI-260 turkey BAC library. The selected markers were distributed on both macro- and microchromosomes and included a genetically unlinked marker. End sequences were obtained for a subset of the recovered BACs and compared to the chicken whole genome sequence. Close association of the turkey BAC-end sequences and original marker sequences was generally conserved in the chicken genome. Gene content of the turkey BACs is predicted from the comparative sequence alignments.
Keywords:BAC  End sequences  Turkey
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