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Short communication: Single nucleotide polymorphisms in an intron of the ovine calpastatin gene |
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| Authors: | B.R. Palmer H.‐Y. Su N. Roberts J.G.H. Hickford R. Bickerstaffe |
| Affiliation: | 1. Animal and Food Sciences Division , Lincoln University , PO Box 84, Canterbury, 8150, New Zealand;2. Animal and Food Sciences Division , Lincoln University , PO Box 84, Canterbury, 8150, New Zealand;3. Division of Molecular Neurobiology , University of Cincinnati College of Medicine , P.O. Box 670559, Cincinnati, OH, 45267, USA E-mail: hung‐yi.su@psychiatry.uc.edu |
| Abstract: | Abstract Calpastatin is the specific inhibitor of the ubiquitous calcium‐dependent proteases μ‐calpain and m‐calpain. Enzyme assay data from sheep and cattle inversely correlates post‐mortem muscle calpastatin levels with ultimate meat tenderness. Genetic markers of meat quality may therefore be found linked to the calpastatin gene (CAST). A three‐allele system detected by polymerase chain reaction ‐ single strand conformational polymorphism (PCR‐SSCP) has been observed in the ovine CAST. The three allele amplimers have been fully nucleotide sequenced and their differences in terms of single nucleotide polymorphism (SNPs) in the intron region of the amplimer are reported and compared to a consensus sequence of the orthologous region of the cattle CAST. A PCR‐RFLP for more rapid CAST genotyping of all three ovine alleles was also developed. |
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