A Single Nucleotide Polymorphism and Sequence Analysis of CSN1S1 Gene Promoter Region in Chinese BOS Grunniens (YAK) |
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| Authors: | W. L. Bai R. H. Yin Q. L. Dou J. C. Yang S. J. Zhao Z. J. Ma |
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| Affiliation: | 1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University , Shenyang, China;2. College of Animal Science and Veterinary Medicine, Jilin University , Changchun, China;3. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University , Shenyang, China;4. College of Agricultural and Animal Husbandry, Qinghai University , Xining, China;5. Institute of Biotechnology, Animal Science Research Academy of Sichuan Province , Chengdu, China;6. Academy of Animal Science and Veterinary Medicine, Qinghai University , Xining, China |
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| Abstract: | The aim of this study was to investigate the polymorphism of the CSN1S1 gene promoter region in 4 Chinese yak breeds, and compare the yak CSN1S1 gene promoter region sequences with other ruminants. A Polymerase Chain Reaction-Single Strand Conformation Polymorphism protocol was developed for rapid genotyping of the yak CSN1S1 gene. One hundred fifty-eight animals from 4 Chinese yak breeds were genotyped at the CSN1S1 locus using the protocol developed. A single nucleotide polymorphism of the CSN1S1 gene promoter region has been identified in all yak breeds investigated. The polymorphism consists of a single nucleotide substitution G→A at position 386 of the CSN1S1 gene promoter region, resulting in two alleles named, respectively, G386 and A386, based on the nucleotide at position 386. The allele G386 was found to be more common in the animals investigated. The corresponding nucleotide sequences in GenBank of yak (having the same nucleotides as allele G386 in this study), bovine, water buffalo, sheep, and goat had similarity of 99.68%, 99.35%, 97.42%, 95.14%, and 94.19%, respectively, with the yak allele A386. |
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| Keywords: | CSN1S1 Polymorphism Yak |
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