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Sex determination of bovine embryos using the polymerase chain reaction |
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| Authors: | A. Schröder J. R. Miller P. D. Thomsen K. Roschlau B. Avery P. H. Poulsen |
| Affiliation: | 1. Dept. of Molecular Genetics , Research Center of Animal Production , Dummerstorf, Dummerstorf‐Rostock, 2551, G.D.R.;2. Dept. of Molecular Genetics , Research Center of Animal Production , Dummerstorf, Dummerstorf‐Rostock, 2551, G.D.R.;3. Department of Molecular Physiology , Institute of Animal Physiology and Genetics Research Babraham , Cambridge, U.K.;4. Department of Animal Genetics , Royal Veterinary and Agricultural University , Copenhagen, Denmark;5. Department of Reproduction , Royal Veterinary and Agricultural University , Copenhagen, Denmark |
| Abstract: | Abstract One or two cell biopsies were obtained from 6‐7 days old bovine embryos. The sex of the embryos was determined with two different bovine Y‐chromosome‐specific primer pairs by using the polymerase chain reaction. These results were confirmed by karyotyping as well as in situ hybridization with an independent bovine Y‐chromosome‐specific sequence. The polymerase chain reaction was found to be a quick and accurate method of sex diagnosis of bovine preimplantation embryos. |
| Keywords: | Chicken A-FABP gene Cloning Tissue expression |