Enhanced Expression of Adenovirus Encoding rhEPO Assisted by BAPTA |
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| Authors: | Zhi-Bin Liu Zeng-Sheng Han Hai Yang Wen-Zong Lu Wen-Ye Li |
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| Affiliation: | 1. College of Animal Science and Technology, Northwest A&2. F University , Yangling, Shaanxi, China;3. Department of Biological Engineering, College of Environment &4. Chemical Engineering , Yanshan University , Qinhuangdao, Hebei, China |
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| Abstract: | Infection efficiency is the key issue for gene delivery using adenovirus vector and usually unsatisfactory. In this study, recombinant adenoviruses encoding recombinant human EPO were prepared using the Adeasy system, and injected into the mammary gland of goats via the teat canal. BAPTA was used to treat the mammary gland to facilitate adenoviruses infection compared with EGTA. Milk serum was collected from the infected mammary gland and characterized by ELISAs and Western blotting. Expression level of rhEPO from the group treated by BAPTA was higher than that treated by EGTA. |
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| Keywords: | Adenovirus BAPTA Erythropoietin Milk serum |
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