The Effect of Retroviral Vector on Uptake of Human Lactoferrin DNA by Yak (Bos Grunniens) Spermatozoa and their Fertilizability In Vitro |
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| Authors: | Xiang-Dong Zi Shao-Wei Chen Guan-Nan Liang Da-Wen Chen Da-Wei Zhang Rong-Hua Yin |
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| Affiliation: | 1. The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities , Chengdu, China zixd2000@yahoo.com;3. The Key Laboratory of Animal Genetics and Breeding, Ministry of Education and State Ethnic Affairs Commission, Southwest University for Nationalities , Chengdu, China |
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| Abstract: | The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using PLNCX2-hLF+FuGene 6 to generate SMGT yak spermatozoa for fertilizing bovine oocytes. The result showed that DNA-binding rate increased with the extension of incubation period and DNA treatment did not decrease sperm motility. Oocytes inseminated with SMGT-spermatozoa had a lower (P < 0.05) cleavage rate (57.7%) than the control (73.4%), but development up to blastocyst stage was not different (26.8 vs. 31.7%). It appears that PLNCX2 is useful for generating transgenic yaks by SMGT. |
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| Keywords: | Human lactoferrin IVF Sperm-mediated gene transfer Yak |
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