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Production of human tissue-type plasminogen activator (htPA) using in vitro cultured transgenic pig mammary gland cells |
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| Authors: | Hak-Jae Chung Hyun-Jung Park Sun-Young Baek Jin-Ki Park Won-Young Lee Kyung-Woon Kim |
| Affiliation: | 1. Swine Science Division, National Institute of Animal Science, Cheoan-si, Republic of Korea;2. Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea;3. Department of Swine &4. Poultry Science, Korea National College of Agriculture and Fisheries, Jeonju, Republic of Korea;5. Department of Beef &6. Dairy Science, Korea National College of Agriculture and Fisheries, Jeonju, Republic of Korea;7. Animal Biotechnology Division, National Institute of Animal Science, Wanju-gun, Republic of Korea |
| Abstract: | AbstractTissue plasminogen activator (tPA) is a protein involved in the breakdown of blood clots. We have previously produced a human tPA (htPA)-overexpressing transgenic pig using a mammary gland-specific promoter. In this study, we have established a transgenic pig mammary gland cell line that produces recombinant htPA. The mammary gland cells grew well and retained their character over long periods of culture. There was no difference in the extent of apoptosis in transgenic cells compared to wild-type mammary gland cells. In addition, the transgenic mammary gland cells expressed and secreted htPA into the conditioned media at a concentration similar to that in milk. This transgenic cell line represents a simple and ethical method for recombinant htPA production. |
| Keywords: | In vitro culture mammary gland cell protein expression tissue-type plasminogen activator (tPA) transgenic pig |