Decreased Expression of Matrix Metalloproteinase-9 and Increased Expression of Tissue Inhibitors of Matrix Metalloproteinase-1 in Paratuberculosis-Infected Cattle in the ELISA-Negative Subclinical Stage |
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| Authors: | Xiaofei Wang Hongyu Wang Aodon-geril Yujing Shu Yuriko Momotani Reiko Nagata |
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| Affiliation: | 1. Research Team for Paratuberculosis, National Institute of Animal Health , Tsukuba, Japan;2. Department of Advanced Medical Science, Division of Cancer Genetics , Nihon University School of Medicine , Tokyo, Japan;3. De Zhou Bureau of Animal Husbandry and Veterinary , People's Republic of China;4. Research Team for Paratuberculosis, National Institute of Animal Health , Tsukuba, Japan |
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| Abstract: | We investigated the gene expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitors of matrix metalloproteinases-1 (TIMP-1) in peripheral blood cells from infected cattle with Mycobacterium avium subsp. paratuberculosis (Map) in the ELISA-negative subclinical stage compared with uninfected control cattle. Significant decreased MMP-9 expression and increased TIMP-1 expression were found in peripheral blood cells from Map-infected cattle after stimulation with Map lysate and Map purified protein derivative (PPD) than in control cattle by real-time RT-PCR analysis. In contrast to the uninfected controls, the activity of MMP-9 was also decreased in peripheral blood cell culture supernatants from Map-infected cattle at 24 hr after Map lysate and MapPPD stimulation by gelatin zymography analysis. As a result, the MMP-9 may play an important role in the development of Mycobacterium avium subsp. paratuberculosis disease. |
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| Keywords: | Cattle Mmp-9 Mycobacterium Paratuberculosis Timp-1 |
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