Isolation and characterization of a lectin from the seeds of Dioclea lehmanni. |
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Authors: | G Perez M Hernandez E Mora |
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Institution: | Department of Chemistry Universidad Nacional, Bogota, Colombia. |
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Abstract: | Affinity chromatography of the globulin fraction from the seeds of Dioclea lehmanni on Sephacryl S-200 yielded two lectins, one slightly retarded and another strongly bound. The latter, which was a glucose/mannose specific lectin, was purified and the following properties were determined: pI, Mr of subunits, carbohydrate content, A, aminoacid composition, hemagglutination and inhibition patterns, N-terminal sequence and mitogenic activity. These properties of the lectin were very similar to those of the Con A and Dioclea grandiflora lectins. |
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