Adaptation of a PAM-fluorometer for remote sensing of chlorophyll fluorescence |
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Authors: | Ounis A. Evain S. Flexas J. Tosti S. Moya I. |
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Affiliation: | (1) Laboratoire pour l'Utilisation du Rayonnement Electromagnétique/CNRS, Bat 203, Centre Universitaire Paris–Sud, B.P. 34, 91898 Orsay cedex, France |
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Abstract: | The Laser-PAM described in this paper is an adaptation of the PAM 101 fluorometer (Heinz Walz, Effeltrich, Germany) designed for remote sensing and non-invasive laboratory measurements of chlorophyll fluorescence. It is based on a 5 mW laser diode, emitting at 638 nm, and a Fresnel lens coupled to the ED-101 detection unit. Due to these modifications, measurements can be performed at a distance ranging from 0.3 to 2 m. The ED-101 detection unit has been modified to perform simultaneous measurements of both modulated fluorescence and light reflected by the leaf. Reflected light showed a good estimation of the photosynthetically active radiation measured exactly at the same area as the fluorescence. A particular advantage of the Laser-PAM fluorometer is its suitability for remote measurements under field conditions. Simultaneous fluorescence and gas-exchange measurements, performed on grapevine leaves, are reported as an example of an application for the Laser-PAM. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | chlorophyll fluorescence laser diode PAM-fluorometer photosynthesis photosynthetically active radiation remote sensing stomatal conductance |
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