Molecular characterization of coding sequences and analysis of Toll-like receptor 3 mRNA expression in water buffalo (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>) and nilgai (<Emphasis Type="Italic">Boselaphus tragocamelus</Emphasis>) |
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Authors: | Animesh Dhara Mohini Saini Dhanjit K Das Devendra Swarup Bhaskar Sharma Satish Kumar Praveen K Gupta |
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Institution: | (1) Centre for Wildlife, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India;(2) Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India;(3) Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India |
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Abstract: | Toll-like receptor 3 (TLR3), an antiviral innate immunity receptor recognizes double-stranded RNA, preferably of viral origin
and induces type I interferon production, which causes maturation of phagocytes and subsequent release of chemical mediators
from phagocytes against some viral infections. The present study has characterized TLR3 complementary DNA (cDNA) in buffalo
(Bubalus bubalis) and nilgai (Boselaphus tragocamelus). TLR3 coding sequences of both buffalo and nilgai were amplified from cultured dendritic cell cDNA and cloned in pGEMT-easy
vector for characterization by restriction endonucleases and nucleotide sequencing. Sequence analysis reveals that 2,715-bp-long
TLR3 open reading frame encoding 904 amino acids in buffalo as well as nilgai is similar to that of cattle. Buffalo TLR3 has
98.6 and 97.9% identity at nucleotide level with nilgai and cattle, respectively. Likewise, buffalo TLR3 amino acids share
96.7% identity with cattle and 97.8% with nilgai. Non-synonymous substitutions exceeding synonymous substitutions indicate
evolution of this receptor through positive selection among these three ruminant species. Buffalo and nilgai appear to have
diverged from a common ancestor in phylogenetic analysis. Predicted protein structures of buffalo and nilgai TLR3 from deduced
amino acid sequences indicate that the buffalo and nilgai TLR3 ectodomain may be more efficient in ligand binding than that
of cattle. Furthermore, TLR3 messenger RNA expression in tissues as quantified by real-time PCR was found higher in nilgai
than buffalo.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. |
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Keywords: | Toll-like receptor 3 Innate immunity Bubalus bubalis Boselaphus tragocamelus Coding sequence |
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