一种改进的双向电泳染色方法

本文涉及了双向电泳过程中的染色方法，即先用考马斯亮蓝染色，将胶上可见蛋白切下再银染的方法。这种方法可最大限度的减少胶中蛋白质点的损失，不仅避免了单一用考马斯亮蓝染色由于灵敏度不高而导致的低丰度蛋白的损失，也避免了单一用银染而使高丰度的蛋白因染色过度导致的损失。同时两种传统的染色方法结合完美，形成的新方法经济实用。

An improved staining method for two-dimensional gel electrophoresis

An improved staining method for two dimensional gel electrophoresis was introduced in this paper. Staining the gel was composed of two steps: firstly to dye with Coomassie brilliant blue and excise protein spots in gel, secondly to perform silver re-staining. This method could increase the number of visible protein spots in gel to great extent, which could not only avoid loss of low-abundance spots in single Coomassie brilliant blue dying gel due to its lower sensitivity, but also avoid loss of high-abundance spots in single silver staining gel due to its higher sensitivity. At the same time Coomassie brilliant blue dying was compatible with silver staining perfectly so it's an effective and economical method.