首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Un vivo depletion of murine CD8 positive T cells impairs survival during infection with a highly virulent strain ofCryptococcus neoformans
Authors:Christopher H Mody  Gwo-Hsiao Chen  Catherine Jackson  Jeffrey L Curtis  Galen B Toews
Institution:(1) Department of Internal Medicine, University of Calgary, Calgary, Alberta, Canada;(2) Division of Pulmonary & Critical Care Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA;(3) Department of Veterans Affairs Medical Center, Ann Arbor, Michigan, USA;(4) Division of Pulmonary Medicine, Room 273 HMRB; 3330 Hospital Drive NW, University of Calgary, T2N 4N1 Calgary, Alberta, Canada
Abstract:Cell-mediated immunity plays an important but incompletely understood role in host defense againstCryptococcus neoformans. Because of their multiple capacities as cytokine-secreting cells, cytotoxic cells, and antigen-specific suppressor cells, CD8 positive T lymphocytes could potentially either enhance or impair host defense againstC. neoformans. To determine whether CD8 T cells enhance or inhibit host defence during an infection with a highly virulent strain ofC. neoformans, we examined the effect of in vivo CD8 cell depletion on suNival and on the number of organisms in mice infected by either the intratracheal or intravenous routes. Adequacy of depletion was confirmed both phenotypically and functionally. Regardless of the route of infection, we found that survival of mice depleted of CD8 T cells was significantly reduced compared to undepleted mice. Surprisingly, however, CD8 depletion did not alter organism burden measured by quantitative CFU assay in mice infected by either route. These data demonstrate that CD8 positive T cells participate in the immune response to a highly virulent strain ofC. neoformans. By contrast to minimally virulent isolates that do not cause a life threatening infection, the immune response to a highly virulent isolate does not alter the burden of organisms, but does enhance host defense as it is necessary for the optimal survival of infected mice.Abbreviations 3H-TdR 3H-thmidine - CFU colony forming units - FITC Fluorescein isothiocyanate - MLR mixed lymphocyte reaction - PBS phosphate buffered saline
Keywords:Cryptococcus  Host defense  Lymphocyte subsets  Mice  Mixed Lymphocyte Reaction
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号