Inhibition of a nuclease contaminant in the commercial preparations of Escherichia coli alkaline phosphatase. |
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| Authors: | M Shinagawa R Padmanabhan |
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| Affiliation: | Department of Biological Chemistry, University of Maryland School of Medicine, 660 West Redwood Street, Baltimore, Maryland 21201 USA |
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| Abstract: | Escherichia coli alkaline phosphatase is a valuable reagent for removal of terminal phosphate from both ribo-and deoxyribo-oligonucleotides or from restriction enzyme fragments of DNA. Some commercial preparations of this enzyme were found to be contaminated with nucleases which could degrade both DNA and RNA. These contaminating nucleases can be completely eliminated by carrying out the enzymic reaction in the presence of 0.1-1% sodium dodecyl sulfate without any loss of phosphatase activity. This report has immediate application in the sequence analysis of DNA or RNA. |
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| Keywords: | To whom reprint requests should be sent. |
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