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Microscale grooves regulate maturation development of hPSC-CMs by the transient receptor potential channels (TRP channels) |
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| Authors: | Taoyan Liu Siyao Zhang Chenwu Huang Shuhong Ma Rui Bai Yanan Li Yun Chang Chenwen Hang Amina Saleem Tao Dong Tianwei Guo Youxu Jiang Wenjing Lu Lina Zhang Luo Jianwen Hongfeng Jiang Feng Lan |
| Affiliation: | 1. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China Contribution: Data curation (lead), Resources (lead);2. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Data curation (equal), Writing - original draft (lead);3. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China Contribution: Data curation (equal), Software (equal);4. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Formal analysis (equal), Validation (equal), Writing - original draft (equal);5. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Formal analysis (equal), Validation (equal);6. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Data curation (equal), Validation (equal), Visualization (equal);7. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Investigation (equal), Validation (equal);8. Department of Cardiology, Peking University Third Hospital, Beijing, China Contribution: Data curation (equal), Validation (equal), Visualization (equal);9. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Data curation (equal), Writing - original draft (equal);10. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China Contribution: Data curation (equal), Validation (equal);11. State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China Contribution: Formal analysis (equal), Software (equal);12. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China Contribution: Data curation (equal), Resources (equal);13. Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Anzhen Hospital, Capital Medical University, Beijing, China;14. Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing, China |
| Abstract: | The use of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is limited in drug discovery and cardiac disease mechanism studies due to cell immaturity. Micro-scaled grooves can promote the maturation of cardiomyocytes by aligning them in order, but the mechanism of cardiomyocytes alignment has not been studied. From the level of calcium activity, gene expression and cell morphology, we verified that the W20H5 grooves can effectively promote the maturation of cardiomyocytes. The transient receptor potential channels (TRP channels) also play an important role in the maturation and development of cardiomyocytes. These findings support the engineered hPSC-CMs as a powerful model to study cardiac disease mechanism and partly mimic the myocardial morphological development. The important role of the TRP channels in the maturation and development of myocardium is first revealed. |
| Keywords: | hPSC-CMs maturation microtopography topographic cues TRP channels |