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PAICS is related to glioma grade and can promote glioma growth and migration |
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| Authors: | Baoshun Du Zheying Zhang Wenyu Di Wenzhong Xu Lei Yang Shitao Zhang Guoyang He Rui Yang Maode Wang |
| Institution: | 1. Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
Second Department of Neurosurgery, Xinxiang Central Hospital, Xinxiang, China;2. Department of Pathology, Xinxiang Medical University, Xinxiang, China;3. Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China;4. Department of Neurosurgery, The Second Hospital Affiliated of Henan University of Science and Technology, Luoyang, China;5. Department of Neurosurgery, The Second Affiliated Hospital of Xi’an Medical University, Xi'an, China;6. Department of Neurosurgery, Xi'an No.3 Hospital, The Affiliated Hospital of Northwest University, Xi'an, China Contribution: Conceptualization (supporting), Formal analysis (supporting), Investigation (supporting), Writing - review & editing (equal);7. Synthetic Biology Engineering Lab of Henan Province, School of Life Science and Technology, Xinxiang Medical University, Xinxiang, China;8. Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China |
| Abstract: | Glioma is a common malignant tumour of the brain. In this study, we aimed to investigate diagnostic biomarkers and its role in glioma. Weighted gene co-expression network analysis (WGCNA) and Cytoscape software were used to screen the marker genes in glioma. RT-qPCR and Western blotting methods were performed to determine the expression of PAICS, ERCC1 and XPA genes in glioma tissues. Expression level of PAICS in different grades of glioma was examined by immunohistochemistry. CCK8 and Colony formation assays were used to detect cell proliferation. Cell adhesion assay was used to detect adhesion ability. Wound healing and transwell tests were used to detect cell migration ability. Flow cytometry was used to detect cell cycle and apoptosis. According to the predicted co-expression network, we identified the hub gene PAICS. Furthermore, we observed that PAICS expression level was up-regulated in glioma tissues compared with normal tissues, and the expression level was correlated with the grade of glioma. Moreover, we found PAICS can promote glioma cells proliferation and migration in vitro. Flow cytometry results showed that si-PAICS cells were stalled at the G1 phase compared with the si-NC cells and knocking down PAICS expression can increase apoptotic rate. PAICS can regulate the mRNA and protein levels of nucleotide excision repair pathway core genes ERCC1 and XPA. l -aspartic acid can affect the expression of PAICS and then inhibit glioma cell proliferation. Our results indicated that PAICS can promote glioma proliferation and migration. PAICS may act as a potential diagnostic marker and a therapeutic target for glioma. |
| Keywords: | glioma grade migration PAICS proliferation weighted gene co-expression network analysis |