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Study of oral microorganisms contributing to non-carious cervical lesions via bacterial interaction and pH regulation |
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| Authors: | Xiaoyu Huang Lin She Huanhuan Liu Pingping Liu Jue Chen Yingcong Chen Wenjie Zhou Youguang Lu Jun Lin |
| Affiliation: | 1. Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Methodology (equal), Validation (equal), Writing - original draft (equal);2. Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Contribution: Investigation (equal), Methodology (equal), Software (equal);3. Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Contribution: Data curation (equal), Formal analysis (equal), Investigation (equal);4. Institute of Applied Genomics, Fuzhou University, Fuzhou, China College of Biological Science and Engineering, Fuzhou University, Fuzhou, China Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, China Contribution: Data curation (equal), Formal analysis (equal), Investigation (equal);5. Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Contribution: Data curation (equal), Software (equal);6. Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China;7. Institute of Applied Genomics, Fuzhou University, Fuzhou, China |
| Abstract: | There is a lack of evidence about the relationship between microorganisms and non-carious cervical lesions (NCCLs) due to limited technologies. A group of 78 patients was enrolled for microbial 16S rRNA sequencing of dental plaques on normal and defective cervical surfaces. Parallel data from 39 patients were analysed with paired t tests, and Fusobacteriales exhibited significantly less distribution on NCCLs than on normal surfaces. As a result, Fusobacterium nucleatum, the most common oral bacterial strain belonging to the order Fusobacteriales, was selected for further research. From a scanning electron microscopy (SEM) scan, the tooth surface with Fusobacterium nucleatum and Streptococcus mutans culture was more intact than that without Fusobacterium nucleatum. Furthermore, the calcium contents in groups with Fusobacterium nucleatum were significantly higher than that without it. In further mechanistic research, Fusobacterium nucleatum was demonstrated to adhere to and disturb other organisms as well as producing alkaline secretions to neutralize the deleterious acidic environment, protecting the tooth structure. In conclusion, microorganisms and NCCLs were confirmed directly related through adherent bacterial interactions and pH regulation. The research provides a new perspective and experimental evidence for the relation between microorganisms and NCCLs, which guides clinical treatment and preventive dentistry in the future. |
| Keywords: | 16S rRNA dental morphology microbial interactions non-carious cervical lesions oral microbiology |