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Activation of formyl peptide receptor 1 elicits therapeutic effects against collagen-induced arthritis |
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| Authors: | Byunghyun Park Mingyu Lee Sang Doo Kim Yu Sun Jeong Ji Cheol Kim Siyoung Yang Hye Young Kim Yoe-Sik Bae |
| Institution: | 1. Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea
Contribution: Conceptualization (equal), Data curation (lead), Formal analysis (lead), ?Investigation (lead), Writing - original draft (lead), Writing - review & editing (equal);2. Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul, Korea Contribution: Formal analysis (supporting), ?Investigation (supporting), Writing - original draft (supporting);3. Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea Contribution: Conceptualization (supporting), ?Investigation (supporting);4. Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea Contribution: Conceptualization (supporting), ?Investigation (supporting), Methodology (supporting);5. Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea Contribution: ?Investigation (supporting), Methodology (supporting);6. Department of Pharmacology, Ajou University School of Medicine, Suwon, Korea;7. Laboratory of Mucosal Immunology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea;8. Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea |
| Abstract: | Rheumatoid arthritis (RA) is an autoimmune disorder which shows production of autoantibodies, inflammation, bone erosion, swelling and pain in joints. In this study, we examined the effects of an immune-modulating peptide, WKYMVm, that is an agonist for formyl peptide receptors (FPRs). Administration of WKYMVm into collagen-induced arthritis (CIA) mice, an animal model for RA, attenuated paw thickness, clinical scores, production of type II collagen-specific antibodies and inflammatory cytokines. WKYMVm treatment also decreased the numbers of TH1 and TH17 cells in the spleens of CIA mice. WKYMVm attenuated TH1 and TH17 differentiation in a dendritic cell (DC)-dependent manner. WKYMVm-induced beneficial effects against CIA and WKYMVm-attenuated TH1 and TH17 differentiation were reversed by cyclosporin H but not by WRW4, indicating a crucial role of FPR1. We also found that WKYMVm augmented IL-10 production from lipopolysaccharide-stimulated DCs and WKYMVm failed to suppress TH1 and TH17 differentiation in the presence of anti-IL-10 antibody. The therapeutic administration of WKYMVm also elicited beneficial outcome against CIA. Collectively, we demonstrate that WKYMVm stimulation of FPR1 in DCs suppresses the generation of TH1 and TH17 cells via IL-10 production, providing novel insight into the function of FPR1 in regulating CIA pathogenesis. |
| Keywords: | collagen-induced arthritis dendritic cell formyl peptide receptor rheumatoid arthritis T cell |