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Analysis of T-cell receptor repertoire in peripheral blood of patients with pancreatic cancer and other pancreatic diseases |
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| Authors: | Hui Wang Yue Yuan Chenglin Lu Siqi Zhou Yixuan Zhang Jing Zhao Chenghu Xu Jie Yang Haochen Su Borui Li Xihan Li Pin Wang Guifang Xu Lei Wang Xiaoping Zou Shanhua Bao Shu Zhang Ying Lv |
| Institution: | 1. Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China;2. Department of Gastroenterology, Nanjing Medical University Affiliated Drum Tower Clinical Medical College, Nanjing, China
Contribution: Data curation (equal);3. Department of General Surgery, The Afflicted Drum Tower Hospital, Nanjing University Medical School, Nanjing, China Contribution: Data curation (equal);4. Department of Gastroenterology, Jiangsu University Affiliated Drum Tower Hospital, Nanjing, China Contribution: Writing - original draft (supporting);5. Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China Contribution: Methodology (supporting);6. Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China Contribution: Software (supporting);7. Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China Contribution: Data curation (supporting);8. Department of Gastroenterology, Nanjing Medical University Affiliated Drum Tower Clinical Medical College, Nanjing, China Contribution: Data curation (supporting);9. Department of Gastroenterology, Nanjing Medical University Affiliated Drum Tower Clinical Medical College, Nanjing, China Contribution: Writing - review & editing (supporting);10. Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China Contribution: Writing - review & editing (supporting);11. Department of General Surgery, The Afflicted Drum Tower Hospital, Nanjing University Medical School, Nanjing, China |
| Abstract: | Pancreatic cancer (PC) has been the fourth cancer-related death worldwide, diagnosed at an unresectable stage due to its rapid progression and few symptoms of this disease at early stages. The aim of this study was to determine the association between the diversity of T-cell receptor (TCR) repertoire and clinicopathological characteristics of patients with PC and other benign pancreatic diseases. In order to make a comprehensive analysis the TCR repertoire, high-throughput sequencing was used to differentiate complementarity determining region 3 (CDR3) of the TCR β chain in peripheral blood samples from 3 PC, 3 chronic pancreatitis, 3 pancreatic cystic lesions and 3 pancreatic neuroendocrine tumour patients. We found that there were significant differences related to TCR repertoire between PC and other pancreatic diseases, and PC is a relatively immunosuppressive tumour. Changes of peripheral TCR repertoire may be used to predict the progression of PC and the response to immunotherapy. And there may exist novel-specific antigens in PC patients which could be used to design targeting immunotherapy in the nearly future. |
| Keywords: | high-throughput sequencing immunotherapy pancreatic cancer pancreatic disease T-cell receptor repertoire |