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Metformin alleviates allergic airway inflammation and increases Treg cells in obese asthma |
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| Authors: | Yimin Guo Jianting Shi Qiujie Wang Luna Hong Ming Chen Shanying Liu Xiaoqing Yuan Shanping Jiang |
| Affiliation: | 1. Department of Respiratory Medicine, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, China Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Resources (equal), Software (equal), Visualization (equal), Writing - original draft (equal);2. Department of Respiratory Medicine, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, China Contribution: Conceptualization (equal), Funding acquisition (equal), Project administration (equal), Supervision (equal);3. Department of Respiratory Medicine, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China;4. Department of Respiratory Medicine, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, China Contribution: Formal analysis (equal), Methodology (equal), Software (equal), Validation (equal);5. Department of Respiratory Medicine, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, China Contribution: Methodology (equal), Software (equal);6. Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Research Center of Medicine, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China Contribution: Conceptualization (equal), Funding acquisition (equal), Project administration (equal), Visualization (equal), Writing - review & editing (equal);7. Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-sen University Second University Hospital, Sun Yat-sen University, Guangzhou, China |
| Abstract: | Obesity increases the morbidity and severity of asthma, with poor sensitivity to corticosteroid treatment. Metformin has potential effects on improving asthma airway inflammation. Regulatory T cells (Tregs) play a key role in suppressing the immunoreaction to allergens. We built an obese asthmatic mouse model by administering a high-fat diet (HFD) and ovalbumin (OVA) sensitization, with daily metformin treatment. We measured the body weight and airway inflammatory status by histological analysis, qRT-PCR, and ELISA. The percentage of Tregs was measured by flow cytometry. Obese asthmatic mice displayed more severe airway inflammation and more significant changes in inflammatory cytokines. Metformin reversed the obese situation and alleviated the airway inflammation and remodelling with increased Tregs and related transcript factors. The anti-inflammatory function of metformin may be mediated by increasing Tregs. |
| Keywords: | Metformin Obese asthma Tregs |