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The identification of fish eggs of two species,the ovate sole Solea ovata and black porgy Acanthopagrus schlegelii
Authors:Yan-jing Zheng  Xiu-qin Li  Zhen-xiong Yang  Wei-xu Cai  Quan-sheng Lou  Wei Tao
Affiliation:1. South China Sea Environmental Monitoring Center, State Oceanic Administration, Guangzhou, China

Key Laboratory of Marine Environmental Survey Technology and Application, Ministry of Natural Resources, Guangzhou, China;2. South China Sea Environmental Monitoring Center, State Oceanic Administration, Guangzhou, China

Abstract:Fish eggs of the ovate sole Solea ovata and black porgy Acanthopagrus schlegelii were identified through DNA barcoding of cytochrome c oxidase subunit I (COX1). Visual taxonomic features were achieved, and photographs of the eggs of both species at different developmental stages were reported for the first time. In addition, the dissolution of oil globules caused by ethanol as egg fixatives was observed. This result showed the importance of using formalin as egg fixatives in the case of morphometric analysis and the necessity of combining molecular and visual taxonomic method for morphological study.
Keywords:Acanthopagrus schlegelii  COX1  ethanol  fish eggs  formalin  Solea ovata
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