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Effect of anticoagulants on farmed giant kokopu,Galaxias argenteus (Gmelin 1789) haematological parameters and erythrocyte fragility |
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| Authors: | Ronald Lulijwa Andrea C. Alfaro Tim Young Leonie Venter Paul Decker Fabrice Merien Jill Meyer |
| Affiliation: | 1. Aquaculture Biotechnology Research Group, School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand National Agricultural Research Organisation, Rwebitaba Zonal Agricultural Research and Development Institute (Rwebitaba-ZARDI), Fort Portal, Uganda;2. Aquaculture Biotechnology Research Group, School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand;3. Aquaculture Biotechnology Research Group, School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand The Centre for Biomedical and Chemical Sciences, School of Science, Auckland University of Technology, Auckland, New Zealand;4. Mahurangi Technical Institute, Manāki Premium New Zealand Whitebait facility, Warkworth, New Zealand;5. Aquaculture Biotechnology Research Group, School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand AUT-Roche Diagnostics Laboratory, School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand;6. AUT-Roche Diagnostics Laboratory, School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand |
| Abstract: | We sought to determine a compatible anticoagulant for routine haematological and physiological assessments with giant kokopu (Galaxias argenteus), an endemic New Zealand fish. We observed that blood treated with lithium heparin (LH) rapidly coagulated and haemolysed, making it unsuitable for G. argenteus. Dipotassium ethylenediaminetetraacetic acid (K2EDTA) and trisodium citrate (citrate) effectively prevented blood coagulation. K2EDTA-treated erythrocytes exhibited the least mean haemolysis and mean corpuscular fragility. Further studies into prolonged storage effects of citrate and K2EDTA are recommended to find a compatible anticoagulant for use with G. argenteus blood. |
| Keywords: | erythrocyte morphology fish blood clotting galaxias argenteus haemolysis osmotic fragility |