Increased UBE2L6 regulated by type 1 interferon as potential marker in TB |
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Authors: | Jiao Gao Chonghui Li Wenjing Li Haotian Chen Yurong Fu Zhengjun Yi |
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Affiliation: | 1. School of Medical Laboratory, Weifang Medical University, Weifang, China;2. School of Medical Laboratory, Weifang Medical University, Weifang, China Contribution: Validation (equal);3. School of Basic Medicine, Weifang Medical University, Weifang, China |
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Abstract: | The aim of this study is to identify potential biomarker of tuberculosis (TB) and determine its function. Differentially expressed mRNAs(DEGs) were selected from a blood database GSE101805, and then, 30 key genes were screened using STING, Cytoscape and further functionally enriched. We then found that only 6 of 13 genes related to ubiquitination (the first in the functional enrichment) were increased significantly. ROC analysis showed that UBE2L6, among 6 genes, had the highest diagnostic value, and then, we found that it also had mild value in differential diagnosis. Moreover, our analysis showed that UBE2L6 may be upregulated by type I interferon, which was further confirmed by us. In addition, we also found that UBE2L6 inhibits the apoptosis of Mycobacterium tuberculosis(Mtb)infected macrophages. Subsequently, we discovered that miR-146a-5p, which may target UBE2L6, is reduced in peripheral blood mononuclear cells (PBMC) and plasma of TB, and it also had certain diagnostic efficiency(AUC=0.791). In brief, we demonstrated that UBE2L6 as well as miR-146a-5p is a potential biomarker for TB and UBE2L6,which may also plays important role in TB by, at least, modulating Mtb-infected macrophage apoptosis. |
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Keywords: | tuberculosis biomarker UBE2L6 miR-146a-5p |
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