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Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway |
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| Authors: | Meiling Zhou Yumei Yuan Zishu Lin Bin Zhang Wenluo Qin Yongqiang Liu Cong Yang Qi Wang Hong Zhang |
| Institution: | 1. Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China;2. Department of Rehabilitation, Shenzhen Bao’An Hospital of Chinese Medicine, Shenzhen, China
Contribution: Methodology (equal), Resources (equal);3. Acupuncture & Rehabilitation Clinical Medicine College, Guangzhou University of Chinese Medicine, Guangzhou, China Contribution: Methodology (equal), Supervision (equal);4. Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China Contribution: ?Investigation (equal), Project administration (equal), Supervision (equal);5. Acupuncture & Rehabilitation Clinical Medicine College, Guangzhou University of Chinese Medicine, Guangzhou, China Contribution: Methodology (equal), Project administration (equal);6. Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, China Contribution: Conceptualization (equal), Methodology (equal), Supervision (equal), Writing - review & editing (equal);7. Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, China Contribution: Formal analysis (equal), Methodology (equal), Supervision (equal);8. Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, China Contribution: Conceptualization (equal), Resources (equal), Supervision (equal);9. Acupuncture & Rehabilitation Clinical Medicine College, Guangzhou University of Chinese Medicine, Guangzhou, China |
| Abstract: | Ageing population is a tough task worldwide, and the aggravating trend of ageing population in China brings enormous pressure to healthcare system. Chinese acupuncture has shown definite anti-ageing effect as arthralgia relief, movement improvement, energy increase and immunity enhancement; however, the mechanisms underlying are far away from illumination. Increasing literature has highlighted the role of alterations in mitochondrial function as a potential central regulator in ageing biology; mitophagy plays a critical role in mitochondrial quality control. In the present study, we demonstrated that acupoint catgut embedding treatment ameliorated ageing-related alterations in appearance, muscle function and spatial memory in rats, reduced degenerated cells in hippocampus, and maintained relatively normal structures in the hippocampus tissue and neurons. These changes were proved to be associated with the regulation of mitochondrial function and autophagic activity. Furthermore, we investigated part of the molecular mechanisms and demonstrated that the PINK1 other than PINK1–Parkin signalling pathway involved in the effects of acupoint catgut embedding, and the imbalancement between mitochondrial fusion and fission and stimulation of mitochondrial biogenesis may aggravate or compensate for impaired mitochondria. The factors act downstream PINK, and the interaction between them for mitochondrial homeostasis in this process remains to be identified. |
| Keywords: | acupoint catgut embedding ageing mitochondria mitophagy PINK1 |