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Primary ciliogenesis is a crucial step for multiciliated cell determinism in the respiratory epithelium |
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| Authors: | Randa Belgacemi Zania Diabasana Antony Hoarau Xavier Dubernard Jean-Claude Mérol Christophe Ruaux Myriam Polette Jeanne-Marie Perotin Gaëtan Deslée Valérian Dormoy |
| Institution: | 1. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France
Contribution: Conceptualization (equal), Formal analysis (equal), Investigation (equal), Writing - original draft (equal), Writing - review & editing (equal);2. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France Contribution: Formal analysis (supporting), Investigation (supporting), Writing - review & editing (supporting);3. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France Contribution: Formal analysis (equal), Investigation (equal), Writing - review & editing (supporting);4. Department of otorhinolaryngology, CHU Reims, Hôpital Robert Debré, Reims, France Contribution: Formal analysis (equal), Resources (equal), Writing - review & editing (supporting);5. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France Department of otorhinolaryngology, CHU Reims, Hôpital Robert Debré, Reims, France Contribution: Formal analysis (equal), Resources (equal), Writing - review & editing (supporting);6. Department of otorhinolaryngology, Clinique Mutualiste La Sagesse, Rennes, France Contribution: Formal analysis (equal), Resources (equal), Writing - review & editing (supporting);7. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France Department of biopathology, CHU Reims, Hôpital Maison Blanche, Reims, France Contribution: Formal analysis (equal), Investigation (equal), Writing - review & editing (equal);8. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France Department of respiratory diseases, CHU of Reims, Hôpital Maison Blanche, Reims, France Contribution: Formal analysis (equal), Investigation (equal), Methodology (equal), Resources (equal), Writing - original draft (equal), Writing - review & editing (equal);9. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France Department of respiratory diseases, CHU of Reims, Hôpital Maison Blanche, Reims, France Contribution: Formal analysis (equal), Investigation (equal), Resources (lead), Supervision (equal), Writing - original draft (equal), Writing - review & editing (equal);10. Université de Reims Champagne-Ardenne, Inserm, UMR-S1250, SFR CAP-SANTE, Reims, France |
| Abstract: | The alteration of the mucociliary clearance is a major hallmark of respiratory diseases related to structural and functional cilia abnormalities such as chronic obstructive pulmonary diseases (COPD), asthma and cystic fibrosis. Primary cilia and motile cilia are the two principal organelles involved in the control of cell fate in the airways. We tested the effect of primary cilia removal in the establishment of a fully differentiated respiratory epithelium. Epithelial barrier integrity was not altered while multiciliated cells were decreased and mucous-secreting cells were increased. Primary cilia homeostasis is therefore paramount for airway epithelial cell differentiation. Primary cilia-associated pathophysiologic implications require further investigations in the context of respiratory diseases. |
| Keywords: | airway epithelium cell differentiation cilia |