|
|||||
|
|
Effect of emodin on long non-coding RNA-mRNA networks in rats with severe acute pancreatitis-induced acute lung injury |
|
| Authors: | Caiming Xu Yalan Luo Michael Ntim Weili Quan Zhaoxia Li Qiushi Xu Liu Jiang Jingwen Zhang Dong Shang Lei Li Guixin Zhang Hailong Chen |
| Institution: | 1. Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China;2. Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China
Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, China Contribution: Methodology (equal), Writing - original draft (supporting);3. Department of Physiology, Dalian Medical University, Dalian, China;4. Center for Genome Analysis, ABLife Inc, Wuhan, China Contribution: Formal analysis (supporting), Methodology (supporting), Writing - original draft (supporting);5. Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, China Contribution: Data curation (equal), Methodology (supporting);6. Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, China Contribution: Methodology (supporting);7. Endoscopy Center, The First Affiliated Hospital of Dalian Medical University, Dalian, China Contribution: Funding acquisition (supporting);8. Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, China Contribution: Supervision (equal), Writing - review & editing (equal);9. Department of Vascular Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China |
| Abstract: | Long non-coding RNAs (lncRNAs) contribute to disease pathogenesis and drug treatment effects. Both emodin and dexamethasone (DEX) have been used for treating severe acute pancreatitis-associated acute lung injury (SAP-ALI). However, lncRNA regulation networks related to SAP-ALI pathogenesis and drug treatment are unreported. In this study, lncRNAs and mRNAs in the lung tissue of SAP-ALI and control rats, with or without drug treatment (emodin or DEX), were assessed by RNA sequencing. Results showed both emodin and DEX were therapeutic for SAP-ALI and that mRNA and lncRNA levels differed between untreated and treated SAP-ALI rats. Gene expression profile relationships for emodin-treated and control rats were higher than DEX-treated and -untreated animals. By comparison of control and SAP-ALI animals, more up-regulated than down-regulated mRNAs and lncRNAs were observed with emodin treatment. For DEX treatment, more down-regulated than up-regulated mRNAs and lncRNAs were observed. Functional analysis demonstrated both up-regulated mRNA and co-expressed genes with up-regulated lncRNAs were enriched in inflammatory and immune response pathways. Further, emodin-associated lncRNAs and mRNAs co-expressed modules were different from those associated with DEX. Quantitative polymerase chain reaction demonstrates selected lncRNA and mRNA co-expressed modules were different in the lung tissue of emodin- and DEX-treated rats. Also, emodin had different effects compared with DEX on co-expression network of lncRNAs Rn60_7_1164.1 and AABR07062477.2 for the blue lncRNA module and Nrp1 for the green mRNA module. In conclusion, this study provides evidence that emodin may be a suitable alternative or complementary medicine for treating SAP-ALI. |
| Keywords: | acute lung injury acute pancreatitis emodin lncRNA mRNA |