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Resveratrol treatment reduces expression of MCP-1, IL-6, IL-8 and RANTES in endometriotic stromal cells |
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| Authors: | Roya Kolahdouz-Mohammadi Farzad Shidfar Sepideh Khodaverdi Tahereh Arablou Sahel Heidari Nesa Rashidi Ali-Akbar Delbandi |
| Affiliation: | 1. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Project administration (lead), Writing - original draft (lead);2. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran Contribution: Conceptualization (equal), Methodology (equal), Writing - review & editing (equal);3. Endometriosis Research Center, Iran University of Medical Science, Tehran, Iran Contribution: Conceptualization (equal), Writing - review & editing (equal);4. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran Contribution: Data curation (equal), Writing - review & editing (equal);5. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Contribution: Data curation (equal), Writing - review & editing (equal);6. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran |
| Abstract: | Endometriosis is an inflammatory disease affecting reproductive-aged women. Immunologic disturbance, as well as inflammation, have crucial roles in the pathogenesis of endometriosis. In this study, we evaluated the effects of resveratrol treatment on expression of monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, and regulated upon activation, normal T cell expressed and secreted (RANTES) in endometrial stromal cells from patients with endometriosis compared with non-endometriotic controls. Thirteen eutopic (EuESCs) and nine ectopic (EESCs) endometrial stromal cells from endometriotic patients as well as eleven endometrial stromal cells from non-endometriotic controls (CESCs) were treated with resveratrol (100 μmol/L) or ethanol, and gene and/or protein expression of MCP-1, IL-6, IL-8 and RANTES was examined at 6, 24 and 48 hours following treatment in the cells from all origins. Resveratrol treatment significantly reduced gene and protein expression of MCP-1, IL-6, and IL-8 in EuESCs and EESCs compared with CESCs (P < .05-.001, P < .05-.001 and P < .05-<.01, respectively), and this reduction was more noticeable in EESCs than EuESCs (P < .05-<.001). Besides, resveratrol treatment significantly reduced RANTES protein expression in EESCs in all time intervals (P < .05). Resveratrol treatment significantly reduced the expression of MCP-1, IL-6, IL-8 and RANTES in EESCs. |
| Keywords: | ectopic endometriosis MCP-1 IL-6 IL-8 RANTES resveratrol stromal cells |