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The association between fat mass and obesity-associated (FTO) genotype and serum vitamin D level in breast cancer patients |
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| Authors: | Maryam Gholamalizadeh Zohreh Mokhtari Saeid Doaei Vahideh Jalili Sayed Hossein Davoodi Mona Jonoush Mohammad Esmail Akbari Azadeh Hajipour Bojlul Bahar Ghasem Azizi Tabesh Saeed Omidi Seyed Alireza Mosavi Jarrahi |
| Institution: | 1. Student Research Committee, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Contribution: Formal analysis (equal);2. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Contribution: Investigation (equal), Supervision (equal);3. Research Center of Health and Environment, Guilan University of Medical Sciences, Rasht, Iran;4. Faculty of Medicine, Urmia University of Medical sciences, Urmia, Iran Contribution: Data curation (equal);5. Departments of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran Contribution: Funding acquisition (equal);6. Department of Nutrition, School of Medicine, Mashahd University of Medical Sciences, Mashahad, Iran Contribution: Software (equal), Validation (equal);7. Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Contribution: Software (equal), Writing - original draft (equal);8. School of Health, Qazvin University of Medical Sciences, Qazvin, Iran Contribution: Investigation (equal), Visualization (equal);9. Nutrition Sciences and Applied Food Safety Studies, Research Centre for Global Development, School of Sport & Health Sciences, University of Central Lancashire, Preston, UK Contribution: Validation (equal), Writing - original draft (equal);10. Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Contribution: Investigation (equal), Visualization (equal);11. Department of Health Education and Promotion, Research Center of Health and Environment, School of Health, Guilan University of Medical Sciences, Rasht, Iran Contribution: Validation (equal), Writing - original draft (equal);12. Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
| Abstract: | The preventive effect of vitamin D against breast cancer can be influenced by gene polymorphisms. This study aimed to investigate the association between serum level of 25(OH) vitamin D and FTO genotype in breast cancer patients. A cross-sectional study was carried out on 180 newly diagnosed patients with breast cancer in Tehran, Iran. The blood samples were collected from the participants in order to assess the FTO gene rs9939609 polymorphism by the tetra-primer amplification refractory mutation system (Tetra-ARMS) PCR method. The serum level of 25(OH) vitamin D was measured using the direct competitive enzyme-linked immunosorbent assay (ELISA) method. The association between vitamin D and the FTO genotype in patients with breast cancer was assessed after adjustment for cofounders. The frequency of TT, AT and AA genotypes in the breast cancer patients were 43% (n = 77), 49% (n = 89) and 8% (n = 14), respectively. All patients with higher than 40 ng/dl of serum 25(OH) vitamin D had one or two copies of FTO rs9939609 risk allele (p = 0.019). No linear association was found between the number of FTO risk allele and the level of serum vitamin D. All patients with high serum level of 25(OH) vitamin D had one or two copies of FTO rs9939609 risk allele. FTO gene polymorphisms may counteract the beneficial effects of vitamin D in breast cancer prevention. Further studies can help to better understand the genetic factors predisposing to breast cancer and their effect on the association between vitamin D and breast cancer. |
| Keywords: | breast cancer FTO gene polymorphism vitamin D |