Pitx2 deletion in pituitary gonadotropes is compatible with gonadal development, puberty, and fertility |
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Authors: | Charles Michael A Mortensen Amanda H Potok Mary Anne Camper Sally A |
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Institution: | Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109-0618, USA. |
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Abstract: | This report introduces a gonadotrope-specific cre transgenic mouse capable of ablating floxed genes in mature pituitary gonadotropes. Initial analysis of this transgenic line, Tg(Lhb-cre)1Sac, reveals that expression is limited to the pituitary cells that produce luteinizing hormone beta, beginning appropriately at e17.5. Cre activity is detectable by a reporter gene in nearly every LHbeta-producing cell, but the remaining hormone-producing cell types and other organs exhibit little to no activity. We used the Tg(Lhb-cre)1Sac strain to assess the role Pitx2 in gonadotrope function. The gonadotrope-specific Pitx2 knockout mice exhibit normal expression of LHbeta, sexual maturation, and fertility, suggesting that Pitx2 is not required for gonadotrope maintenance or for regulated production of gonadotropins. |
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Keywords: | Cre recombinase homeobox gene luteinizing hormone beta subunit transgenic mice floxed allele reproduction conditional knockout |
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