Genotyping single nucleotide polymorphisms by primer extension and high performance liquid chromatography |
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| Authors: | Bastiaan Hoogendoorn Michael J Owen Peter J Oefner Nigel Williams Jehannine Austin M C O’Donovan |
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| Institution: | (1) Division of Psychological Medicine, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, UK e-mail: odonovanmc@cardiff.ac.uk, Tel.: +44-1222-743242, Fax: +44-1222-747839, GB;(2) Department of Genetics, Stanford University, Stanford, CA 94305, USA, US;(3) Division of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, UK, GB |
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| Abstract: | We have investigated the possibility of genotyping single nucleotide polymorphisms (SNPs) by primer extension and high performance
liquid chromatography (HPLC). Using three polymorphisms of current interest to our group (an A/G polymorphism in the proneurotensin
gene and A/G and T/C polymorphisms in the 5HT2a receptor gene), we show that robust signal is obtained using this simple analytic
method which has the added advantages that sample loading and analysis are essentially automated, analytic time is brief,
and no further purification step after primer extension is required. We also show that all stages of the HPLC-primer extension
genotyping can be multiplexed which, together with automation, suggests that this system may be suitable for linkage studies
based upon emerging SNP maps.
Received: 27 April 1998 / Accepted: 26 November 1998 |
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