渗透压在葡萄酒酵母代谢中的调控作用

以磷酸丙糖异构酶部分缺失突变株做对比,研究了渗透压对葡萄酒酵母发酵过程中甘油合成与挥发酸生成的调节作用.结果表明:渗透压对野生型葡萄酒酵母中存在的磷酸二羟丙酮(DHAP)与3-磷酸甘油醛(GA3P)平衡具有调节作用,能使平衡向磷酸二羟丙酮方向迁移以合成更多的甘油,而当磷酸丙糖异构酶部分缺失时渗透压对这一平衡基本不起作用...

Osmotic Press Plays an Important Role in Metabolic Control of Saccharomyces ellipsoideus

Using triose phosphate isomerase deficient (tpiΔ) mutant of Saccharomyces ellipsoideus as the control, the regulation of osmotic press on the balance between glycerol production and volatile acid formation was first studied. The results demonstrated that there existed the control of osmotolerance on the balance between dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) in wild S. ellipsoideus and the suitable high osmotolerance can transport metabolic flux from GA3P to DHAP to produce much glycerol. However, the control of osmotolerance didn't exist in tpiΔ mutant of S. ellipsoideus. In comparison with wild S. ellipsoideus, the tpiΔ mutant greatly reduced the yield of glycerol production and much created glycolytic metabolites because of GA3P accumulation at the same conditions. Therefore, the conclusion was made that, at the suitable high osmotolerance, DHAP accumulation of wild S. ellipsoideus would be helpful to regulate the ratio of glycerol to volatile acid.