毛白杨PtSEP2基因启动子克隆和瞬时表达特性分析

利用PCR技术从毛白杨基因组DNA中扩增获得花器官发育相关的SEPALLATA2类似基因PtSEP25′侧翼约2.3kb的一段序列,经PlantCARE序列分析表明,该序列中含有启动子特征的保守序列及多种光应答元件,初步推测其为PtSEP2基因启动子.进一步以GUS为报告基因,构建了pPtSEP2 promoter::...

Cloning and Transient Expression Analysis of PtSEP2 Promoter from Populus tomentosa

A 5' flanking sequence of PtSEP2 which is a SEPALLATA2 -like gene involved in floral organ development was amplified by PCR from the genomic DNA of Populus tomentosa. The length of fragment is approximately 2.3kb. The results derived from PlantCARE analysis showed that the sequence contains conserved cis-acting elements of promoter, coupled with a variety of light-responsive elements. Therefore, it was speculated preliminarily to be the promoter of PtSEP2 gene. To investigate function of this promoter, it was fused to GUS reporter gene, generating a plant expression vector pPtSEP2 promoter∷GUS, named PtSEP2p∷GUS . With roots, stems, leaves and flower buds of Nicotiana tobaccum for the receptor, the results of transient expression that Agrobacterium-mediated that showed the PtSEP2 promoter was competent to drive specially GUS reporter gene expression in anther, yet its’ activity was weaker than that of cauliflower mosaic virus (CaMV) 35S promoter, which constitutively expressed in planta. Consequently, provides a possible genetic modification tool for flowering regulation in poplar and other plants.