| p38蛋白激酶参与BMP9诱导的C3H10T1/2细胞成骨分化 |
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| 引用本文： | 徐道晶,王锦,何娟文,胡晶,翁亚光,罗进勇.p38蛋白激酶参与BMP9诱导的C3H10T1/2细胞成骨分化[J].中国生物工程杂志,2011,31(5):15-21. |
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| 作者姓名： | 徐道晶 王锦 何娟文 胡晶 翁亚光 罗进勇 |
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| 作者单位： | 重庆医科大学医学检验系 重庆医科大学检验医学教育部重点实验室 重庆市重点实验室 重庆 400016 |
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| 基金项目： | 国家自然科学基金，重庆市科委自然科学基金 |
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| 摘 要： | 目的:初步分析丝裂原活化蛋白激酶p38在BMP9诱导间充质干细胞C3H10T1/2成骨分化过程中的作用.方法:利用BMP9重组腺病毒感染C3H10T1/2细胞,Western blot检测p38激酶总蛋白表达水平和磷酸化水平.p38的特异性抑制剂SB203580抑制p38活性或RNA干扰抑制p38表达后,分析ALP活性...
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| 关 键 词： | 骨形态发生蛋白9 间充质干细胞 p38 成骨分化 |
| 收稿时间： | 2010-12-23 |
| 修稿时间： | 2011-02-28 |
p38 Kinase Participated in BMP9-induced Osteogenic Differentiation of C3H10T1/2 Mesenchynal Stem Cells |
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| XU Dao-jing,WANG Jin,HE Juan-wen,HU Jing,WENG Ya-guang,LUO Jin-yong.p38 Kinase Participated in BMP9-induced Osteogenic Differentiation of C3H10T1/2 Mesenchynal Stem Cells[J].China Biotechnology,2011,31(5):15-21. |
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| Authors: | XU Dao-jing WANG Jin HE Juan-wen HU Jing WENG Ya-guang LUO Jin-yong |
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| Institution: | XU Dao-jing WANG Jin HE Juan-wen HU Jing WENG Ya-guang LUO Jin-yong(Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective:Analysis the functional role of p38 kinase in BMP-9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.Methods:C3H10T1/2 cells were infected by recombinant adenovirus expressing BMP9,then the total protein level and phosphorylated form of p38 kinase were determined by Western blot.After treatment C3H10T1/2 cells with p38 specific inhibitor SB203580 or using RNA interference to silence expression of p38,the early osteogenic marker ALP activity was detected by quantitative and st... |
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| Keywords: | BMP9 Mesenchymal stem cells p38 Osteogenic differentiation |
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