| HMGB1 siRNA 对HT-29 细胞侵袭转移能力的影响 |
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| 引用本文： | 刘彦华 雷宁 韩威 曾庆乐 刘俊达. HMGB1 siRNA 对HT-29 细胞侵袭转移能力的影响[J]. 现代生物医学进展, 2011, 11(11): 2110-2112 |
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| 作者姓名： | 刘彦华 雷宁 韩威 曾庆乐 刘俊达 |
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| 作者单位： | 1. 成都医学院医学检验系,四川,成都,610083
2. 成都理工大学,材料与化学化工学院,化工与制药系,四川,成都,610059 |
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| 摘 要： | 目的:观察干扰HMGB1表达对HT-29细胞侵袭转移能力的影响。方法:HMGB1 siRNA通过脂质体转染HT-29细胞,western blot和实时定量RT-PCR检测HT-29细胞中HMGB1蛋白和mRNA的表达,Transwell小室观察HT-29的转移侵袭能力。结果:干扰HMGB1后HMGB1蛋白和mRNA的表达均减少,HT-29的转移侵袭能力下降。结论:HMGB1能促进HT-29的转移侵袭能力,干扰其表达可抑制HT-29的转移侵袭。
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| 关 键 词： | HMGB1 HT-29 侵袭力 |
Effects of High Mobility Group Box-B1 on Invasive Ability of HT-29 |
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| LIU Yan-hu,LEI Ning,HAN Wei,ZENG Qing-le,LIU Jun-da. Effects of High Mobility Group Box-B1 on Invasive Ability of HT-29[J]. Progress in Modern Biomedicine, 2011, 11(11): 2110-2112 |
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| Authors: | LIU Yan-hu LEI Ning HAN Wei ZENG Qing-le LIU Jun-da |
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| Affiliation: | 1 Faculty of Laboratorial Examination,Chengdu Medical College Chengdu 610083; 2 Department of Chemical & Pharmaceutical Engineering,College of Material and Chemistry & Chemical Engineering,Chengdu University of Technology.Chengdu 610059) |
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| Abstract: | Objective: To study the effect of HMGB1on invasive ability of HT-29. Methods: HT-29 cells were divide into blankgroup, control group and HMGB1 siRNA groups. The effects of HMGB1 siRNA on HT-29 cells invasive ability in vitro was measuredby Boyden chamber invasive models. Contents of HMGB1 protein and mRNA were detected by western blot and realtime RT-PCRmethods, respectively. Result: In HMGB1 siRNA group, contents of HMGB1 protein and mRNA were decreased markedly, the invasiveability was decreased markedly, too. Conclusion: HMGB1 could increase the invasive ability of HT-29 cell. |
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| Keywords: | HMGB1 HT-29 Invasive ability |
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