| 白藜芦醇对乳腺癌细胞MCF-7 增殖的影响 |
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| 引用本文： | 王毅铮,臧照辉 牛秀珑 张岭 王越 陈虹.白藜芦醇对乳腺癌细胞MCF-7 增殖的影响[J].现代生物医学进展,2011,11(12):2254-2257. |
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| 作者姓名： | 王毅铮 臧照辉 牛秀珑 张岭 王越 陈虹 |
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| 作者单位： | 1. 武警医学院,天津,300162;天津市职业与环境危害生物标志物重点实验室,天津,300162
2. 武警医学院,天津,300162 |
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| 摘 要： | 目的:研究白藜芦醇体外活性,确定它的植物雌激素作用。方法:采用MTT法观察不同浓度白藜芦醇对MCF-7细胞增殖作用的影响。采用DNA ladder法和荧光显微镜观察高浓度白藜芦醇对细胞的影响。免疫组化法观察低浓度白藜芦醇对核增殖抗原PCNA表达的影响。结果:MTT结果显示白藜芦醇高浓度抑制MCF-7细胞增殖,IC50为8.70×10-5mol/L;低浓度(10-7~10-6mol/L)则对细胞有促增殖作用,最高促增殖浓度为1.0×10-7mol/L。DNA ladder和荧光显微镜可观察到高浓度白藜芦醇作用后细胞典型的凋亡形态。免疫组化结果显示低浓度白藜芦醇作用后,细胞核内PCNA表达明显增加(P<0.05)。结论:高、低浓度的白藜芦醇对MCF-7细胞分别表现为诱导凋亡和促增殖作用,呈现出植物雌激素对MCF-7细胞典型的双向调节作用。
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| 关 键 词： | 植物雌激素 白藜芦醇 MCF-7细胞 增殖 |
Effects of Resveratrol on Proliferation of MCF-7 Cells in Breast Cancer |
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| WANG Yi-zheng,ZANG Zhao-hui,NIU Xiu-long,ZHANG Ling,WANG Yue,CHEN Hong.Effects of Resveratrol on Proliferation of MCF-7 Cells in Breast Cancer[J].Progress in Modern Biomedicine,2011,11(12):2254-2257. |
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| Authors: | WANG Yi-zheng ZANG Zhao-hui NIU Xiu-long ZHANG Ling WANG Yue CHEN Hong |
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| Institution: | WANG Yi-zheng1,2,ZANG Zhao-hui1,NIU Xiu-long1,ZHANG Ling1,WANG Yue1,CHEN Hong1,2(1 Medical College of Chinese People's Armed Police Forces,300162,Tianjin,China,2 Tianjin Key Laboratory of Biomarkers for Occupational and Environmental Hazard,China) |
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| Abstract: | Objective:Our goals were to examine the phytoestrogen-like effect of Resveratrol(RES) using in vitro tests.Methods:Effects of RES on proliferation of MCF-7 cells were detected by MTT test.The fluorescent microscopes by the staining of Hoechst 33342 and DNA gel electrophoresis analysis were used to determine the effects of MCF-7 cells by RES.Immunohistochemistry was used to assess PCNA expression.Results:A MTT test and growth curve showed that the proliferation of MCF-7 cells was inhibited by a high concentr... |
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| Keywords: | Phytoestrogen Resveratrol MCF-7 cell Proliferation |
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