NF-kB 拮抗剂PDTC 对HepG2 细胞的抑制及对caspase-3 表达的影响

目的:研究NF-kB拮抗剂PDTC诱导人肝癌HepG2细胞凋亡及其对凋亡相关基因caspase-3表达的影响,初步探讨其诱导凋亡的可能机制。方法:以不同浓度的PDTC处理人肝癌HepG2细胞,利用MTT法检测对人肝癌HepG2细胞凋亡的影响;利用RT-PCR和Western-blot检测caspase-3mRNA和蛋白的表达。结果:不同浓度PDTC作用人肝癌HepG2细胞不同时间后,能够显著抑制HepG2细胞的生长增殖,存在剂量和时间依赖性(P<0.05);PDTC能够上调HepG2细胞中caspase-3mRNA和蛋白的表达。结论:NF-kB拮抗剂PDTC对人肝癌HepG2细胞产生显著抑制,并能上调HepG2细胞中caspase-3mRNA和蛋白的表达。

Effect of NF-kB Antagonist PDTC in Inhibition of Human Hepatic Carcinoma Cell HepG2

Objective: To investigate the role of apoptosis by PDTC,the selective inhibit of NF-kB on human hepatic carcinoma cell (HepG2). and the effect of expression of csaspase-3,principium disguisition the likelymechalism which induce HepG2 cells apoptosis. Methods: After treating human hepatic carcinoma cell (HepG2) by PDTC, MTT assay was used to observe the growth inhibition of cell- HepG2. The mRNA and protein expression of caspase-3 were detected by RT-PCR and Western-blot assay, respectively. Results: When PDTC was given, growth inhibition and elevated apoptosis of HepG2 cells were detected,which was showed in a dose- and time- - dependent manner, PDTC up-regulated the lever of mRNA and protein expression of caspase-3. Conclusion: PDTC can induce apoptosis of human hepatic carcinoma cell HepG2 cells,which may be performed by up-regulated of caspase-3 expression.