| 吲哚胺2,3-双加氧酶基因转染对肝癌细胞凋亡的影响及相关机制研究 |
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| 引用本文： | 卜晓倩,张瑞,申慧琴,罗静,刘燕,张路英,刘春亮,王琦.吲哚胺2,3-双加氧酶基因转染对肝癌细胞凋亡的影响及相关机制研究[J].中国生物工程杂志,2011,31(5):22-27. |
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| 作者姓名： | 卜晓倩 张瑞 申慧琴 罗静 刘燕 张路英 刘春亮 王琦 |
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| 作者单位： | 山西医科大学 太原 030001 |
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| 基金项目： | 山西省科研攻关资助项目 |
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| 摘 要： | 目的:通过细胞培养和在体实验探讨吲哚胺2,3-双加氧酶(indoleamine 2,3-dioxygenase,IDO)基因转染后对肝癌细胞凋亡的影响及相关细胞免疫机制的研究.方法:提取健康人外周血中的T细胞利用细胞培养和基因转染技术将T细胞和肝癌细胞混合培养.实验分为6组:根据是否加入D-1-MT分为未干预组和干预组...
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| 关 键 词： | 吲哚胺2 3双加氧酶 肝癌细胞 T细胞 Treg细胞 凋亡 |
| 收稿时间： | 2010-12-28 |
| 修稿时间： | 2011-02-22 |
Effects of Hepatocellular Carcinoma Cells'Apoptosis and the Related Mechanisms after Indoleamine 2,3-Dioxygenase Gene Transfection |
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| BU Xiao-qian,ZHANG Rui,SHENG Hui-qin,LUO Jing,LIU Yan,ZHANG Lu-ying,LIU Chun-liang,WANG Qi.Effects of Hepatocellular Carcinoma Cells'Apoptosis and the Related Mechanisms after Indoleamine 2,3-Dioxygenase Gene Transfection[J].China Biotechnology,2011,31(5):22-27. |
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| Authors: | BU Xiao-qian ZHANG Rui SHENG Hui-qin LUO Jing LIU Yan ZHANG Lu-ying LIU Chun-liang WANG Qi |
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| Institution: | BU Xiao-qian ZHANG Rui SHENG Hui-qin LUO Jing LIU Yan ZHANG Lu-ying LIU Chun-liang WANG Qi(Shanxi Medical University,Taiyuan 030001,China) |
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| Abstract: | Objective:To explore after indoleamine-2,3-dioxygenase(IDO)gene transfection the influence of the hepatocellular carcinoma cells' apoptosis and the related cellular immune mechanisms by cell culture and in vivo.Methods: By cell culture and gene transfection technology,T-lymphocytes freshly isolated from healthy people and hepatocellular carcinoma cells were cocultured.The experiments were divided into six groups: T-lymphocytes and HepG2 cells group,T-lymphocytes and pcDNA3.1-HepG2 cells group,T-lymphocytes ... |
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| Keywords: | Indoleamine 2 3-dioxygenase Hepatocellular carcinoma cells T-lymphocyte Treg cells Apoptosis |
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