| RMP基因干扰对肝癌细胞周期的影响 |
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| 引用本文： | 郭云兰,杨慧翠,连晓宁,曹锴,王晓婷,仲蕾,魏文祥.RMP基因干扰对肝癌细胞周期的影响[J].中国生物工程杂志,2011,31(5):8-14. |
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| 作者姓名： | 郭云兰 杨慧翠 连晓宁 曹锴 王晓婷 仲蕾 魏文祥 |
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| 作者单位： | 苏州大学基础医学与生物科学学院 苏州 215123 |
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| 摘 要： | 目的:建立RMP(RPB5-Mediating Protein)基因干扰的稳定细胞株,研究RMP基因干扰对正常肝细胞及其肝癌细胞周期的影响.方法:构建RMP基因的短发卡RNA(short-hairpin RNA,shRNA)真核表达载体pGPU6-Neo-RMPi-484.通过脂质体转染的方法转染到SMMC-7721肝...
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| 关 键 词： | 干扰 RMP 肝癌细胞 细胞周期 |
| 收稿时间： | 2010-09-03 |
| 修稿时间： | 2010-10-18 |
Effects of RPB5-mediating Protein(RMP)on Cell Cycle of Hepatoma Cells |
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| GUO Yun-lan,YANG Hui-cui,LIAN Xiao-ning,CAO Kai,WANG Xiao-ting,ZHONG Lei,WEI Wen-xiang.Effects of RPB5-mediating Protein(RMP)on Cell Cycle of Hepatoma Cells[J].China Biotechnology,2011,31(5):8-14. |
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| Authors: | GUO Yun-lan YANG Hui-cui LIAN Xiao-ning CAO Kai WANG Xiao-ting ZHONG Lei WEI Wen-xiang |
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| Institution: | GUO Yun-lan YANG Hui-cui LIAN Xiao-ning CAO Kai WANG Xiao-ting ZHONG Lei WEI Wen-xiang(College of Basic Medicine and Biological Sciences,Soochow University,Suzhou 215123,China) |
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| Abstract: | Obiective:To study the effects and mechanisms of RMP on cell cycle of human hepatoma cells SMMC-7721.Methods:Construct the shRNA recombinant eukaryotic expression vector pGPU6-Neo-RMP.Establish shRNA RMP stable cell lines;RT-PCR was used to detect the interference efficacy against RMP gene;Flow cytometry was applied to measure the effect of RMP on cell cycle of SMMC-7721,and then the expressions of related genes of cell cycle were detected by RT-PCR.Results: The shRNA recombinant eukaryotic expression vecto... |
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| Keywords: | RNA interference RMP Hepatoma cell Cell cycle |
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