直接注射法制作ICR 小鼠肝癌原位移植瘤模型

目的:培养鼠肝癌H22细胞,直接注射法制作ICR小鼠肝癌原位移植瘤,为后续实验奠定基础。方法:鼠肝癌H22细胞体外培养,将调整好的对数生长期的肝癌细胞直接注射小鼠肝脏,2周后解剖观察,并进行组织HE染色。结果:所有实验小鼠均可见肿瘤生长,HE染色示肝细胞肝癌。结论:直接注射法制作ICR小鼠肝癌原位移植瘤模型简便易行,值得推广应用。

Making an Orthotopic Tumor Model of Hepatocellular Carcinoma forICR Mice by Direct Injection

Objective: To cultivate H22 cell line and making an orthotopic tumor model of hepatocellular carcinoma on ICRmouse using direct injection method to lay the foundation for subsequent experiment. Methods: Cultivate H22 cell line in vitro and anorthotopic xenograft tumor model of hepatocellular carcinoma was created by injection of H22 cells in logarithmic growth phase directlyinto the liver parenchyma of ICR mice. Two weeks later, hepatocellular carcinoma specimens of animals were observed and stained withhematoxylin/eosin. Results: Tumor growth happened on all the experimental mice and was showed as hepatocellular carcinoma byhematoxylin-eosin staining. Conclusion: It is convenient of direct injection method on making an orthotopic tumor model ofhepatocellular carcinoma and should be reported and used widely.