| 利用高通量生物反应器优化一种CHO细胞无血清培养基 |
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| 引用本文： | 陈先金,彭雯丹,郭欣泳,陈小佳,谢秋玲.利用高通量生物反应器优化一种CHO细胞无血清培养基[J].生物学杂志,2011,28(5):86-90. |
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| 作者姓名： | 陈先金 彭雯丹 郭欣泳 陈小佳 谢秋玲 |
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| 作者单位： | 暨南大学,生物工程研究所,广东省生物工程药物重点实验室,广州,510632 |
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| 基金项目： | 广东省产学研项目"杭体规模制备技术及肿瘤杭体药物Herceptin的研发，暨南大学科研培育与创新基金研究项目-11610503 |
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| 摘 要： | 研究以DMEM/F12(1:1 V/V)培养基为基础,添加不同添加剂优化一种适宜CHO DG44细胞生长的廉价培养基。以细胞密度和细胞活率为主要指标,对DMEM/F12(1:1 V/V)培养基进行了优化。通过正交试验和单因素试验筛选出了CHO DG44细胞生长的最佳培养基。正交试验结果表明添加8mg/L Insulin、10mg/L Transferrin、12mM Glutamine、9mg/L Ethanolamine、9mg/L Sodium selenite、0.5×Lipids、0.5×Vitamin,对细胞生长有较好促进作用,细胞密度从0.6×106 cells/mL上升到1.8×106 cells/mL。在此基础上添加2.5g/L Malt Peptone和2.5g/L YeastExtract可使细胞密度达到2.65×106 cells/mL,基本上达到商业培养基的培养效果,而成本降低了约60%。
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| 关 键 词： | CHO细胞 无血清培养基 高通量生物反应器 |
Developement of a serum-free media for CHO cells using a high-throughput bioreactor |
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| CHEN Xian-jin,PENG Wen-dan,GUO Xin-yong,Chen Xiao-jia,XIE Qiu-lin.Developement of a serum-free media for CHO cells using a high-throughput bioreactor[J].Journal of Biology,2011,28(5):86-90. |
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| Authors: | CHEN Xian-jin PENG Wen-dan GUO Xin-yong Chen Xiao-jia XIE Qiu-lin |
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| Institution: | CHEN Xian-jin,PENG Wen-dan,GUO Xin-yong,Chen Xiao-jia,XIE Qiu-lin(Bioengineering Institute,Jinan University,Key Lab of Bioengineering Medicine in Guangdong Province,Guangzhou 510632,China) |
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| Abstract: | In order to get a cheap medium for CHO cells,different supplements were added to DMEM/F12 medium.It was found that cell density could be increased from 0.6×106 cells/mL to 1.8×106 cells/mL with addition of 8mg/L insulin,10mg/L transferrin,12mM glutamine,9mg/L ethanolamine,9mg/L sodium selenite,0.5 lipids and 0.5 vitamin.By supplying 2.5g/L malt peptone and 2.5g/L yeast extract,cell density reached 2.65×106 cells/mL,which was as same as cell density in commercial medium. |
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| Keywords: | CHO cells serum-free medium high-throughput bioreactor |
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