| 利用噬菌体肽库筛选与HIV-1p24抗原结合的多肽 |
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| 引用本文： | 王子晔,冯娟,马雪梅,王明连,钟儒刚.利用噬菌体肽库筛选与HIV-1p24抗原结合的多肽[J].中国生物工程杂志,2011,31(5):104-107. |
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| 作者姓名： | 王子晔 冯娟 马雪梅 王明连 钟儒刚 |
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| 作者单位： | 北京工业大学生命科学与生物工程学院 北京 100124 |
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| 基金项目： | 北京市"中青年骨干教师培养计划"资助项目 |
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| 摘 要： | 通过噬菌体展示技术筛选出与HIV-1p24抗原结合的多肽,为用多肽辅助p24抗原检测提供实验基础.以重组p24抗原为靶蛋白,对噬菌体随机七肽库进行三轮筛选,用EUSA鉴定第三轮筛选到的噬菌体克隆与p24重组抗原的结合能力,再对噬菌体克隆进行测序分析,同时研究了ELISA中噬菌体加入量及多种封闭剂对噬菌体特异性结合能力的...
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| 关 键 词： | 噬菌体展示 肽HIV-1 p24 |
| 收稿时间： | 2010-12-20 |
| 修稿时间： | 2011-02-22 |
Screening of HIV-1 p24 Binding Peptides from Phage Display Peptide Library |
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| WANG Zi-ye,FENG Juan,MA Xue-mei,WANG Ming-lian,ZHONG Ru-gang.Screening of HIV-1 p24 Binding Peptides from Phage Display Peptide Library[J].China Biotechnology,2011,31(5):104-107. |
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| Authors: | WANG Zi-ye FENG Juan MA Xue-mei WANG Ming-lian ZHONG Ru-gang |
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| Institution: | (College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China) |
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| Abstract: | A phage display peptide library was screened by targeting recombinant p24 antigen. After three rounds of panning, eleven phage clones were chosen to test the binding affinity by ELISA, and ten of them were positive. DNA sequencing showed that nine of the positive clones display a unique sequence FTTRANA which has high affinity with HIV-1 p24 antigen. To improve the condition of phage ELISA, phage number and blocking buffer have been optimized, phage number ranged from 8.13×1011 to 1.02×1014 pfu/well and 0.5%BSA was highly recommended. Therefore, in this study, peptides combined with p24 antigen were obtained which might be useful in p24 antigen assay. |
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| Keywords: | Phage display Peptide HIV-1 p24 |
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