牛肠激酶催化亚基工程菌培养条件的探索与鉴定

目的:探索牛肠激酶催化亚基工程菌高效表达的培养条件。方法:牛肠激酶催化亚基基因的初步表达,筛选构建工程菌,从以下方面入手优化培养条件:培养基的组成、摇瓶发酵培养条件、培养基的PH、种龄、接种量、培养时间等,并且通过Western Blotting和SDS-PAGE等鉴定不同条件下的实验结果,从而确定最佳培养条件。结果:通过鉴定实验结果,从不同种平行培养条件中选择产量最高的培养条件,作为最优培养条件。结论:成功地探索并鉴定了工程菌的适宜培养条件。

Exploration and Identification of SuitableCulture Conditions for Bovine Enterokinase Catalytic Subunit Engineering Bacteria

Objective: To investigate the suitable cultivate condition of the high expression of bovine enterokinase catalytic subunit engineering bacteria. Methods: The bovine enterokinase catalytic subunit preliminary was expressed and the engineering bacteria was constructed. The cultivate condition was optimized from the following aspects: medium culture conditions of the optimal composition, wave bottle fermentation cultivation condition, culture medium PH , seed age, seed number, culture time. The Western Blotting and SDS-PAGE identification to grope the most appropriate culture condition. Results: The highest yield cultivative conditions were choice from the same parallel culture conditions as a optimal cultivation condition. Conclusions: The culture condition of bovine enterokinase catalytic subunit engineering bacteria had been optimized successfully.