A novel neisserial shuttle plasmid: a useful new tool for meningococcal research |
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| Authors: | O' Dwyer Clíona A Langford Paul R Kroll J Simon |
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| Affiliation: | Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, Norfolk Place, London W2 1PG, UK. c.odwyer@imperial.ac.uk |
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| Abstract: | We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research. |
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| Keywords: | Plasmid Neisseria meningitidis Shuttle vector |
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