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Production of Alkaline Protease with Teredinobacter turnirae in Controlled Fed-batch Fermentation
Authors:Usama?Beshay  author-information"  >  author-information__contact u-icon-before"  >  mailto:ugabra@hotmail.com"   title="  ugabra@hotmail.com"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author,Antonio?Moreira
Affiliation:(1) Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology Applications, New Bourg El-Arab City, Research Area, 21934 Alexandria, Egypt;(2) Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (UMBC), 21250 Baltimore, MD, USA
Abstract:By using our previously optimized media and a fed-batch operation controlled by LabVIEW Software, the key parameter for a high production of alkaline protease using the marine bacterium, Teredinobacter turnirae, was to maintain a low concentration of C and N-sources ( < 2 g sucrose l−1 and < 0.2 g NH4Cl l−1) using an appropriate fed-batch culture system. A maximum protease activity of 8250 U ml−1 was thus achieved.
Keywords:alkaline protease  fed-batch culture  Teredinobacter turnirae
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