红胫戟纹蝗痘病毒形态及理化性质研究

红胫戟纹蝗Dociostaurus kraussi是新疆草原优势种蝗虫。1989年首次从新疆玛纳斯红胫戟纹蝗上分离到痘病毒Dociostaurus kraussi ntomopoxvirus(DkEPV)，1992年又在新疆巴里坤发现， 自然流行率达23.3%。显微镜观察表明该病毒主要感染脂肪体。病毒球状体为圆球状，直径为2—7μm，大小差异悬殊，病毒粒子砖形或椭圆形，表面呈桑椹结构， 大小平均为144nlnx269nn。病毒DNA具有典型的核酸紫外吸收光谱。根据热变性曲线测得DkEPV—DNA的T_m，值为79.0，(G+C)%为23.7%。病毒DNA经限制性内切酶EcoRI、Bgl IIH和Hind III酶切后，分别得到29、21和18个片段。以λDNA Hind III酶切片段为标准分子量，计算出各酶切片段的分子量为155.45x10⁶、155.69x10⁶和155.40x10⁶D， 由此得出DkEPV—DNA总分子量为55.5x10⁶D。

SOME CHARACTERISTICS OF DOCIOSTAURUS KRA USSIENTOMOPOXVIRUS IN XINJIANG UYGUR AUTONOMOUS REGION

Dociostaurus kraussi is the dominant species in Xinjiang rangeland. In 1989, Dociostauras kraussi entomopoxvirus (DkEPV) was first isolated from Dociostaurus kraussi in Manas,Xinjiang Automonous Region. In 1992, it was found in Barkol, Xinjiang, too. The natural epidemic rate of it was 23.3 %. DkEPV was found to infect mainly the fat body. Occlusion bodies are spheroidal with diameter of 2-7μm, very different in size. The virions obtained from the occlusion body are brick-shaped or ovoid, 144- 269 nm in size. The surface of a virion has a mulberry-like structure. DkEPV showed the typical ultraviolet absorption spectrum of nucleic acid. The Tm ofDkEPV-DNA is about 79.0Y3 based on the thermal denaturation curve, and its G+C content is about 17.8 %. Restriction endonuclease analysis reveals that EcoR I, Bgl II and Hind III digestedthe DkEPV-DNA into 29, 21 and 18 DNA fragments, respectively. Taking λDNA Hind III fragments as standard for molecular weight measurement, the molecular weights of these DNA fragments are 155. 45 x 10⁶ 155.69 x 10⁶ and 155.40 x 10^{6 daltons, repectively, and the total molecular weight is 155.5 x 106 daltons.}