棉铃虫乙酰胆碱酯酶(AChE)的体躯分布及部分纯化

该文研究了棉铃虫Helicoverpa armigera乙酰胆碱酯酶(AChE)的体躯分布及部分纯化技术。在幼虫和成虫体内均以头部AChE活性最高；AChE在成虫和幼虫头、胸、腹部的活性分布分别为78.2%、3.9%、17.9%和90.3%、6.5%和3.2%。通过用Sepharose 4B和Sephadex G-200纯化后，AChE的最高纯化倍数为3.66和17.74倍。样品和洗脱液中含有Triton X-100时，纯化倍数明显高于无Triton X-100的处理组。

DISTRIBUTION AND PURIFICATION OF ACETYLCHOLINESTERASE INCOTYON BOLLWORM (LEPIDOPTERA: NOCTUIDAE)

Body distribution and partial purification of acetylcholinesterase (ACHE) were investigated in cotton bollworm, Helicoverpa armigera (H(ibner). The head, thorax and abdomen possessed 78.2 96, 17.9 96 and 3.9 96 of total ACHE activities in the 7th-day adult, while in the sixth instar larva, the percentages were 90.3 %, 6.5 % and 3.2 % respectively. Eighteen times of the purified factors in ACHE ctivities were obtained by Sephardex G-200, but only four times by Sepharose 4B in column chromatography. The purified factors of ACHE were higher with Triton X-l00 than without Triton X-100 in eluant.