意大利蝗痘病毒一些特性研究

电镜观察意大利蝗痘病毒的包涵体大多数为椭圆形，少数为近圆形，大小相差极为悬殊。病毒粒子呈卵圆形，表面桑椹状。病毒粒子髓核呈圆筒状，其纵切面内绳索结构折叠2—3次，横切面可以看到2—3个圆点，侧体也圆筒状，均匀包围在核衣壳外面。DNA经限制性内切酶LoR I酶切产生16个DNA片段，据此求得DNA分子量为135.7×106D。 意大利蝗痘病毒包涵体的裂解释放病毒速度快，在相同条件下比亚洲小车蝗痘病毒包涵体裂解释放病毒粒子快。当温度恒定(37℃)，在一定的pH值内(pH 9.0—11.5)，随pH值的升高包涵体裂解释放病毒粒子速度加快。当pH值恒定(11.2)，在一定的温度范围内(25～40℃)，随温度的升高包涵体的裂解释放病毒粒子速度加快。

SOME CHARACTERISTICS OF CALLIPTAMUS ITALICUSENTOMOPOXVIRUS IN XINJIANG UYGUR AUTONOMOUS REGION

With electron microscope, most of the occlusion bodies of CiEPV are ellipictal in shape, some are square-round. They vary greatly in size. The virions are oval and mulberry-like in appearance. The core of the virion is cylindrical. From the longitudinal section , its inner ropelike structure folds back 2 or 3 times and from its cross section, 2 or 3 dots can be observed. The lateral body is also cylindrical and symmetrically encloses the nucleocapsid. This viral DNA, when cleaved with restriction enzyme EcoR I, produces 16 DNA fragments.The molecular weight of this DNA is 135.7×10⁶ daltons. Degradation of the occlusion body of CiEPV is faster than that of OaEPV under the same condition. When the temperature is constant at 37℃ and pH ranges within 9-11.5, with the higherpH, the degradation of occlusion body becomes faster. When the pH is constant at 11.2 and the temperature ranges within 25-40℃, with the higher temperature, the degradation of occlusion body becomes faster.