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Trifluoperazine and other anaesthetics inhibit rat liver CTP: phosphocholine cytidylyltransferase
Authors:S L Pelech  F Jetha  D E Vance
Institution:Institute of Biochemistry, Academy of Sciences, Armenian SSR, Yerevan 375044, USSR
Abstract:Chlorpromazine (25 microM) and trifluoperazine (25 microM) inhibited by 5-fold the activity of CTP:phosphocholine cytidylyltransferase, the rate-limiting enzyme for phosphatidylcholine biosynthesis, in rat liver cytosol. Addition of saturating amounts of rat liver phospholipid to the enzyme assay rapidly reversed the drug-mediated inhibition. Three-fold or greater concentrations of these drugs were required to produce a 50% inhibition of the microsomal cytidylyltransferase. Incubation of rat hepatocytes with 20 microM trifluoperazine or chlorpromazine did not inhibit phosphatidylcholine biosynthesis. These results provide additional evidence for the hypothesis that the active form of cytidylyltransferase is on the endoplasmic reticulum and the enzyme in cytosol appears to be latent.
Keywords:Benzylamine oxidase  Brain microvessel  Copper-containing enzyme
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